LOCUS       pLV-[hsa-miR-1257]        9171 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7092..7952
                     /label=Amp(R)
     promoter        8153..8693
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5697..5931
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6506..6961)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6003..6133
                     /label=SV40 pA
     misc_feature    6134..6347
                     /label=SV40 ori
     misc_feature    5018..5614
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3636
                     /label=intron
     CDS             3683..5008
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3426
                     /label=hsa-mir-1257 precursor
BASE COUNT     2148 a      2399 c      2513 g      2111 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg atgaggagga 
     3121 cacttggaac caggcattgg atgcgagccc caccccatgg gagggggacg gggggatgct 
     3181 gggcaccagg gggacctgtg ggccagctgg ccctgggctt gtgcttgggg agtgaatgat 
     3241 gggttctgac ccccatgcac ccctgtgggc ccctggcatc actggcccca tccttcaccc 
     3301 ctgccaacca cgcttgccct gtgcctctga agctccccca ctaagggggt ggagtctaat 
     3361 tccctgtccc ttgactgtgg gttcagccat gtgacttgct ttgactgagg ggatgttcac 
     3421 ggggcagcta gctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat 
     3481 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg 
     3541 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct 
     3601 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact acccctctag 
     3661 agtcgagcta ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat 
     3721 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 
     3781 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 
     3841 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 
     3901 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 
     3961 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 
     4021 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 
     4081 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 
     4141 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 
     4201 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 
     4261 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 
     4321 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 
     4381 gctgtacaag gacccaccgg tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc 
     4441 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     4501 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     4561 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     4621 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     4681 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     4741 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     4801 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     4861 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     4921 cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg 
     4981 gtgcatgacc cgcaagcccg gtgcctgagc ggccgcgtcg acaatcaacc tctggattac 
     5041 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 
     5101 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 
     5161 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 
     5221 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 
     5281 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 
     5341 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     5401 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 
     5461 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 
     5521 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 
     5581 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac 
     5641 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     5701 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 
     5761 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     5821 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     5881 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     5941 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     6001 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     6061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     6121 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc 
     6181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     6241 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     6301 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 
     6361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 
     6421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 
     6481 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     6541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     6601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     6661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     6721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     6781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     6841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     6901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 
     6961 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     7021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     7081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     7141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     7201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     7261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     7321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     7381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     7441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     7501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     7561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     7621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     7681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     7741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     7801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     7861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     7921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     7981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     8041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     8761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     8821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     8881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     8941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     9001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
     9061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
     9121 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg c 
//
